SUMMARY OF SNP - February, 2013 (Pass #4)

Classification of SNP: Large branch. L1335 is a son of DF13 and has three known sons: L743, L1065 and L1334. This analysis is now less speculative in nature since the nature of the curve of the testing candidates suggests overlap with non-L1335 submissions is better known with 42 submissions out of 650 good testing candidates have tested positive. Note that the L1065 fingerprint is currently the same as L1335-M (L1335-P tested negative for L1065).

Scope of Testing within Fingerprint (67 markers): With over 650 testing candidates, much more exhaustive testing is required to determine how much overlap of positive and negative submissions exists at lower fingerprint matches. Analysis continues to support one primary fingerprint and a second very small fingerprint. Broad testing outside of these two fingerprints is still warranted but should be considered somewhat speculative since over 100 submissions have now been tested.

Dominant Surnames (positive): Many surnames.

Source of SNP: FTDNA - WTY testing FTDNA ID 48641, Y-Search ID KKVJF, Surname MacMilan, discovered November, 2012.

Number of Positive Tests (67 markers): Forty-seven known.

Number of Negative Tests below 50 % matches (67 markers): Seventy-four known.

Number of Negative Tests within Fingerprints (higher than 70 %): None.

Tests between 50 and 70 % matches (67 markers): Two positive and one negative to date.

Pending Tests (within Fingerprint): Probably many.

TRENDS OF TESTING CANDIDATES

L1335 Fingerprints

With the addition of the H4 >= 12 marker to the L1335-M fingerprint, the number of known testing candidates now appears to be more typical for L21 SNPs where the number of testing candidates declines down to 70 % matches, remains low between 50 and 70 % and then sees a sharp rise as non-L1335 submissions dominate the under 50 % matches. L1335-M will probably test positive for almost all submissions down to 70 % matches, have a significant mixture of positive and negative test results between 50 to 70 % matches and then test only negative for below 50 % matches. Below is a chart that summarizes the primary fingerprint:

ISOGG Status - Prove relationship of L1335 to other L21 SNPs

Requirements of proving L1335 position on the ISOGG haplotree have been met and L1335 has been added to the ISOGG haplotree. Below is a summary of SNPs tested.

ISOGG Status - Prove genetic and surname diversity of L1335

L1335 satisfies both the 15 % genetic diversity requirement and the surname diversity requirement for inclusion on the ISOGG haplotree. Two submissions of L1335 must have at least a genetic distance of 10 (15 % x 67 markers = 10).

DNA FINGERPRINT & SIGNATURES

Note: All fingerprints and signatures are now much more analyzed but could require fine tuning as additional testing reveals the true scope of L1335. Currently, there remains only two fingerprints associated with L1335 and broad testing may yet discover more fingerprints.

L1335-M Fingerprint (L21 Off Modal Mutations):

Markers 1 to 37: 391 <= 10, 389-2 >= 30, 458 >= 18, H4 >= 12 and YCAIIb >= 24 (5 mutations).

Markers 38 to 67: 531 >= 12; 413a <= 22 and 444 <= 11 (3 mutations).

Note: 458 >18 and GATA-H4 >= 12 have more than one-half of the submissions with the above L21 off-modal marker values. However, there remains a significant quantity of submissions that do not have the off-modal marker values. It is not really possible to determine if the MRCA of the L1335 is the L21 modal value or that these mutations were post-L1335 in origin.

L1335 Signature (R-L21 Plus): 1335-SC

Markers 1 to 37: 391 <= 10, 389-2 >= 30, 449 >= 30, H4 = 12 and YCAII >= 24/22 (4 mutations).

Markers 38 to 67: 531 >= 12; 413a <= 22 and 444 <= 11 (3 mutations).

L1335-P Fingerprint (L21 Off Modal Mutations):

Markers 1 to 37: 391 <= 10, 385a >= 12, 459a >= 10, 448 <= 18, 449 <= 29, 464b >= 16, 464c <= 16, H4 >= 12, 456 <= 15, 607 >= 16 and 576 >= 19 (11 mutations).

Markers 38 to 67: 511 >= 11; 534 <= 14, 444 >= 13 and 446 <= 12 (4 mutations).

L1335 Signature (R-L21 Plus): 1335-W2

Markers 1 to 37: 391 <= 10, 448 <= 18, 464 = 15,16,17,17 and 456 <= 15 (4 mutations).

Markers 38 to 67: (0 mutations).

Note: 464 should be 15,16,16,17